User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/08/31: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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#Fluorescence data run every 5 minutes for 3 hours | #Fluorescence data run every 5 minutes for 3 hours | ||
<u> | <u>Solutions for 09/06/2016</u><br> | ||
Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours. | Solutions were added to glass test tubes, covered with foil, and placed in an oven set to 80°C for 4 hours. | ||
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### 604 μL Lysozyme | ### 604 μL Lysozyme | ||
### 1871 μL Water | ### 1871 μL Water | ||
#Fluorescence Summary Graphs | |||
[[Image:Fluorescence Emission 08312016 Lysozyme pH4.png | thumb | upright=4.0]]<br> | |||
'''Figure 1''' Maximum emission data of tryptophan in a solution of pH 4. Red points correspond to the times shown in '''Figure 3'''. | |||
[[Image:083116 Integ Fluor pH 4.png| thumb | upright=4.0]] | |||
'''Figure 2''' Integrated fluorescence data of tryptophan in a solution of pH 4. Red points correspond to the times shown in '''Figure 3'''. | |||
[[Image:0831016 Full Spectra pH4.png | thumb | upright=4.0]]<br> | |||
'''Figure 3''' Emission summary of important points at t=0, 5, 35, 75, and 175. | |||
<u>'''Figure 3''' Explanation</u><br> | |||
#At t=0 min, we see a max emission far to the left of the other emission spectra. | |||
#At t=5 min, this maximum has shifted up and to the right. | |||
#Until t=35 min, the maximum for each spectra decreases until the shown maximum. | |||
#After t=35 min, the maximum begins increasing again. | |||
#At t=75 min, the maximum continues to increase, but the "shoulder" seen at around 475 nm begins going away. | |||
#t=175 shows the final full spectra obtained. | |||
<u>Solution Prep</u> | <u>Solution Prep</u> | ||
'''These were the initial solutions made with the wrong concentration of lysozyme. See the last 2 tables for the correct solutions.''' | |||
Lysozyme UVVis solution measurement 0 mM fructose | Lysozyme UVVis solution measurement 0 mM fructose | ||
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| 4375 | | 4375 | ||
|} | |} | ||
< | |||
Below are the correct solutions re-made by Dr. Hartings<br> | |||
<u>stock solutions</u> | |||
# AuCl<sub>3</sub> stock A (from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/30|8/30]]) | |||
## mass = 10.13 mg | |||
## 10.0 mL volumetric flask | |||
## concentration = 3.34 mM | |||
# AuCl<sub>3</sub> stock B (from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/31|8/31]]) | |||
## mass = 12.6 mg | |||
## 10.0 mL volumetric flask | |||
## concentration = 4.17 mM | |||
# Fructose (from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/30|8/30]]) | |||
## mass = 23.48 mg | |||
## 10.0 mL volumetric flask | |||
## concentration = 13.0 mM | |||
# Lysozyme stock 1 (from [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/08/31|8/31]]) | |||
## mass = 8.88 mg | |||
## 10.0 mL volumetric flask | |||
## concentration = 62.1 μM | |||
# Lysozyme stock 2 (made today) | |||
## mass = 21.92 mg | |||
## 10.0 mL volumetric flask | |||
## concentration = 153 μM | |||
{|border="1" cellpadding="2" | |||
|+ 0 mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HCl<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AuCl<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of BSA<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 300<sup>B</sup> || 0 || 1006<sup>1</sup> || 3194 | |||
|- | |||
| 5 || 50 || 0 || 0 || 300<sup>B</sup> || 0 || 1006<sup>1</sup> || 3644 | |||
|- | |||
| 6 || 5 || 0 || 0 || 300<sup>B</sup> || 0 || 1006<sup>1</sup> || 3689 | |||
|- | |||
| 7 || 0 || 0 || 0 || 300<sup>B</sup> || 0 || 1006<sup>1</sup> || 3694 | |||
|- | |||
| 8 || 0 || 5 || 0 || 300<sup>B</sup> || 0 || 408<sup>2</sup> || 4287 | |||
|- | |||
| 9 || 0 || 50 || 0 || 300<sup>B</sup> || 0 || 408<sup>2</sup> || 4242 | |||
|- | |||
| 10 || 0 || 500 || 0 || 300<sup>B</sup> || 0 || 408<sup>2</sup> || 3792 | |||
|- | |||
| 11 || 0 || 0 || 5 || 300<sup>B</sup> || 0 || 408<sup>2</sup> || 4287 | |||
|- | |||
| 12 || 0 || 0 || 50 || 300<sup>B</sup> || 0 || 408<sup>2</sup> || 4242 | |||
|} | |||
{|border="1" cellpadding="2" | |||
|+ 0.0625 mM Fructose | |||
! scope="col" width="50" | pH | |||
! scope="col" width="100" | μL of 1 mM HCl<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 mM NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of 1 M NaOH<sub>stock</sub> | |||
! scope="col" width="100" | μL of AuCl<sub>3stock</sub> | |||
! scope="col" width="100" | μL of Fructose<sub>stock</sub> | |||
! scope="col" width="100" | μL of BSA<sub>stock</sub> | |||
! scope="col" width="100" | μL of Water | |||
|- | |||
| 4 || 500 || 0 || 0 || 300<sup>B</sup> || 24 || 408<sup>2</sup> || 3768 | |||
|- | |||
| 5 || 50 || 0 || 0 || 300<sup>B</sup> || 24 || 408<sup>2</sup> || 4218 | |||
|- | |||
| 6 || 5 || 0 || 0 || 300<sup>B</sup> || 24 || 408<sup>2</sup> || 4263 | |||
|- | |||
| 7 || 0 || 0 || 0 || 300<sup>B</sup> || 24 || 408<sup>2</sup> || 4268 | |||
|- | |||
| 8 || 0 || 5 || 0 || 300<sup>B</sup> || 24 || 408<sup>2</sup> || 4263 | |||
|- | |||
| 9 || 0 || 50 || 0 || 374<sup>A</sup> || 24 || 408<sup>2</sup> || 4144 | |||
|- | |||
| 10 || 0 || 500 || 0 || 374<sup>A</sup> || 24 || 408<sup>2</sup> || 3694 | |||
|- | |||
| 11 || 0 || 0 || 5 || 374<sup>A</sup> || 24 || 408<sup>2</sup> || 4189 | |||
|- | |||
| 12 || 0 || 0 || 50 || 374<sup>A</sup> || 24 || 408<sup>2</sup> || 4144 | |||
|} | |} | ||
*'''[[User:Matt Hartings|Matt Hartings]] 12:52, 2 September 2016 (EDT)''': I have taken the complementary data for a sample with no gold. Please see data [[User:Matt Hartings/Notebook/AU Biomaterials Design Lab/2016/09/02|here]] | |||
__NOTOC__ | __NOTOC__ |
Latest revision as of 01:54, 27 September 2017
Project name | Main project page Next entry | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
Objectives
DescriptionFluorescence
Solutions for 09/06/2016
DataFluorescence
Figure 1 Maximum emission data of tryptophan in a solution of pH 4. Red points correspond to the times shown in Figure 3. Figure 2 Integrated fluorescence data of tryptophan in a solution of pH 4. Red points correspond to the times shown in Figure 3. Figure 3 Emission summary of important points at t=0, 5, 35, 75, and 175. Figure 3 Explanation
Solution Prep These were the initial solutions made with the wrong concentration of lysozyme. See the last 2 tables for the correct solutions. Lysozyme UVVis solution measurement 0 mM fructose
Lysozyme UVVis solution measurement .0625 mM fructose
Below are the correct solutions re-made by Dr. Hartings
|