User:Hannah Harvey/Notebook/CHEM-471 Fall 2016

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Objectives

  1. Run UV-vis for 18 samples with lysozyme as the protein. Full concentration details can be found here or below under Data.

Description

  1. Test solutions made according to these concentrations on 8/31 or below under Data.
  2. On 8/31, solutions were placed into the oven at 80°C for 4 hours.
  3. UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations.
  4. After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H2O, twice with HCl, and twice more with H2O. The cuvette was then dried thoroughly.

Data

Solution concentrations
stock solutions

  1. AuCl3 stock A (from 8/30)
    1. mass = 10.13 mg
    2. 10.0 mL volumetric flask
    3. concentration = 3.34 mM
  2. AuCl3 stock B (from 8/31)
    1. mass = 12.6 mg
    2. 10.0 mL volumetric flask
    3. concentration = 4.17 mM
  3. Fructose (from 8/30)
    1. mass = 23.48 mg
    2. 10.0 mL volumetric flask
    3. concentration = 13.0 mM
  4. Lysozyme stock 1 (from 8/31)
    1. mass = 8.88 mg
    2. 10.0 mL volumetric flask
    3. concentration = 62.1 μM
  5. Lysozyme stock 2 (from 9/01)
    1. mass = 21.92 mg
    2. 10.0 mL volumetric flask
    3. concentration = 153 μM
0 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 300B 0 10061 3194
5 50 0 0 300B 0 10061 3644
6 5 0 0 300B 0 10061 3689
7 0 0 0 300B 0 10061 3694
8 0 5 0 300B 0 4082 4287
9 0 50 0 300B 0 4082 4242
10 0 500 0 300B 0 4082 3792
11 0 0 5 300B 0 4082 4287
12 0 0 50 300B 0 4082 4242


0.0625 mM Fructose
pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water
4 500 0 0 300B 24 4082 3768
5 50 0 0 300B 24 4082 4218
6 5 0 0 300B 24 4082 4263
7 0 0 0 300B 24 4082 4268
8 0 5 0 300B 24 4082 4263
9 0 50 0 374A 24 4082 4144
10 0 500 0 374A 24 4082 3694
11 0 0 5 374A 24 4082 4189
12 0 0 50 374A 24 4082 4144

Figure 1 Absorbance v wavelength of lysozyme in 0.0 mM concentration of fructose


Figure 2 Absorbance at 527 nm at various pH's in 0.0 mM concentration of fructose


Figure 3 Absorbance v wavelength of lysozyme in 0.0625 mM concentration of fructose


Figure 4 Absorbance at 527 nm at various pH's in 0.0627 mM concentration of fructose

Notes

All Figures in the above data are baseline and water corrected.

Recent changes

30 November 2016

+ N    14:30 User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/29‎ (2 changes) . . (+1,530) . . (Page history) [Hannah Harvey‎ (2×)]
+      14:06 User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/08‎ (2 changes) . . (0) . . (Page history) [Hannah Harvey‎ (2×)]
+ N    13:53 User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/16‎ (3 changes) . . (+3,023) . . (Page history) [Hannah Harvey‎ (3×)]
       13:39 User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/15 (diff; hist) . .  (+1,484) . . Hannah Harvey (Talk | contribs)
       13:31 User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/02 (diff; hist) . .  (0) . . Hannah Harvey (Talk | contribs)

29 November 2016

  N    14:20 User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/11/15 (diff; hist) . .  (+945) . . Hannah Harvey (Talk | contribs) (Autocreate 2016/11/15 Entry for User:Hannah_Harvey/Notebook/CHEM-471_Fall_2016)


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