Objective
Samples for group A
Make samples using the stock solutions I prepped yesterday.
Stock solutions for groups B and C experiments
Make stock solutions of lysozyme and myoglobin for experiments
Description
Samples for group A
Solutions were added to glass test tubes, covered with aluminum foil, and placed in an oven (set to 80°C) for 4 hours.
Data
Stock solutions
- Lysozyme
- 8.88 mg of lysozyme with water in 10.0 mL volumetric flask
- MW = 14307 g/mol
- concentration = 62.1 μM
- Myoglobin
- 13.71 mg of myoglobin with water in 10.0 mL volumetric flask
- MW = 16951 g/mol
- concentration = 80.9 μM
- AuCl3
- 12.6 mg of AuCl3 with water in 10.0 mL volumetric flask
- MW = 303.3 g/mol
- concentration = 4.17 mM
Instructions for making samples for kinetics experiments
- pH 4
- Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
- 225 μL AuCl3
- 300 μL 1M HCl
- 604 μL Lysozyme
- 1871 μL Water
- Myoglobin: 0.25mM Au, 12.5μM Myoglobin, 3mL total
- 225 μL AuCl3
- 300 μL 1M HCl
- 464 μL Myoglobin
- 1871 μL Water
AuNP synthesis results
pH 4 through 12 (left to right), 0.25 mM Au, 3.1 μM BSA, 0 mM fructose
pH 4 through 12 (left to right), 0.25 mM Au, 3.1 μM BSA, 0.0625 mM fructose
Notes
This area is for any observations or conclusions that you would like to note.
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Sarah Burkhard
Patrick Hampson
Hannah Harvey
Anneliese Faustino 160831
User:Sara K. Mattson/Notebook/Chemistry 671/2016/08/31
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