User:Hannah Harvey/Notebook/CHEM-471 Fall 2016/2016/09/06

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Objectives Run UV-vis for 18 samples with lysozyme as the protein. Full concentration details can be found here or below under Data. Description Test solutions made according to these concentrations on 8/31 or below under Data. On 8/31, solutions were placed into the oven at 80°C for 4 hours. UV-vis data was run on each of the 18 samples of different pHs and fructose concentrations. After each individual sample was run, the sample was saved and the cuvette cleaned out twice with soap, twice with H2O, twice with HCl, and twice more with H2O. The cuvette was then dried thoroughly. Data Solution concentrations stock solutions AuCl3 stock A (from 8/30) mass = 10.13 mg 10.0 mL volumetric flask concentration = 3.34 mM AuCl3 stock B (from 8/31) mass = 12.6 mg 10.0 mL volumetric flask concentration = 4.17 mM Fructose (from 8/30) mass = 23.48 mg 10.0 mL volumetric flask concentration = 13.0 mM Lysozyme stock 1 (from 8/31) mass = 8.88 mg 10.0 mL volumetric flask concentration = 62.1 μM Lysozyme stock 2 (from 9/01) mass = 21.92 mg 10.0 mL volumetric flask concentration = 153 μM 0 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water 4 500 0 0 300B 0 10061 3194 5 50 0 0 300B 0 10061 3644 6 5 0 0 300B 0 10061 3689 7 0 0 0 300B 0 10061 3694 8 0 5 0 300B 0 4082 4287 9 0 50 0 300B 0 4082 4242 10 0 500 0 300B 0 4082 3792 11 0 0 5 300B 0 4082 4287 12 0 0 50 300B 0 4082 4242 0.0625 mM Fructose pH μL of 1 mM HClstock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AuCl3stock μL of Fructosestock μL of BSAstock μL of Water 4 500 0 0 300B 24 4082 3768 5 50 0 0 300B 24 4082 4218 6 5 0 0 300B 24 4082 4263 7 0 0 0 300B 24 4082 4268 8 0 5 0 300B 24 4082 4263 9 0 50 0 374A 24 4082 4144 10 0 500 0 374A 24 4082 3694 11 0 0 5 374A 24 4082 4189 12 0 0 50 374A 24 4082 4144 Figure 1 Absorbance v wavelength of lysozyme in 0.0 mM concentration of fructose Figure 2 Absorbance at 527 nm at various pH's in 0.0 mM concentration of fructose Figure 3 Absorbance v wavelength of lysozyme in 0.0625 mM concentration of fructose Figure 4 Absorbance at 527 nm at various pH's in 0.0627 mM concentration of fructose Notes All Figures in the above data are baseline and water corrected.