Lidstrom:Protocols: Difference between revisions
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*[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]] | *[[Lidstrom: Agarose Gel Electrophoresis|Agarose Gel Electrophoresis]] | ||
*[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | *[[Lidstrom:Sequencing with GeneWiz|Sequencing with GeneWiz]] | ||
====Mutagenesis==== | |||
*[[Lidstrom:EMS Mutagenesis|EMS Mutagenesis]] | |||
*[[Lidstrom:UV Mutagenesis|UV Mutagenesis]] | |||
====Misc.==== | ====Misc.==== | ||
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*[[Lidstrom:French Press|French Press]] | *[[Lidstrom:French Press|French Press]] | ||
*[[Lidstrom:Sonicator|Sonication]] | *[[Lidstrom:Sonicator|Sonication]] | ||
*[[Lidstrom:Bug Buster|Bug Buster]] | |||
*[[Lidstrom:Ultracentrifuge|Ultracentrifuge]] | *[[Lidstrom:Ultracentrifuge|Ultracentrifuge]] | ||
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===General=== | ===General=== | ||
*[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]] | *[[Lidstrom:Enzyme Assay Basics|Enzyme Assay Basics]] | ||
*[[Lidstrom:Enzyme Assay Data Analysis|Enzyme Assay Data Analysis]] | |||
*[[Lidstrom:Solution Stock Info|Solution Stock Info]] | *[[Lidstrom:Solution Stock Info|Solution Stock Info]] | ||
*[[Lidstrom:Path Length in microwell plates|Path Length in 96-well plates]] | *[[Lidstrom:Path Length in microwell plates|Path Length in 96-well plates]] | ||
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==== Misc ==== | ==== Misc ==== | ||
*[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]] | *[[Lidstrom:Assaying enzyme libraries in 96-well plates|Assaying enzyme libraries in 96-well plates]] | ||
*[[Lidstrom:Assaying enzyme libraries|Assaying enzyme libraries]] | |||
== Metabolomics == | == Metabolomics == | ||
Revision as of 12:58, 5 August 2014
Back home: Lidstrom
General Lab:
- Health & Safety
- Finding Chemicals
- Dishwasher
- Autoclave
- Sterile technique
- E. Coli basics
- Preparing Freezer Cell Stocks
- Pouring Media Plates
- Filtration
Cell Culture:
Methylotrophs
E. coli
OD measurements
DNA/RNA:
DNA
PCR
Isolation
- QIAquick Gel Extraction Kit Protocol
- Genomic DNA minikit extraction
- Genomic DNA phenol/chloroform extraction
Manipulation
Fancy Manipulation
- Site-Directed Mutagenesis (in progress)
- λ-Red Mediated Gene Knockouts (in progress)
- Phage Lysate Prep
- P1 Phage Transduction (in progress)
Restriction Cloning
Gibson Cloning
Obervation
Mutagenesis
Misc.
- Loading Dye Recipe
- Purifying DNA (in progress)
- Miniprep (in progress)
- Oligo Orders
- Diluting Primers
- NEB cloning guide
Protein
Purification
Analytics
Protein Concentration Determination
- Choosing a protein concentration quantification method
- BCA assay to determine protein concentration
- NanoDrop for proteins
Chemicals
- Chemical Stability
- Also, see Solution Stock Info
Buffers
E. coli
E. coli transformation
Lysing & Cells & Clarifying Extract
Assays
General
- Enzyme Assay Basics
- Enzyme Assay Data Analysis
- Solution Stock Info
- Path Length in 96-well plates
- Reducing Agents
Equipment
- Plate readers:
- Cuvette spectrophotometer:
- Centrifuge:
- Sorvall centrifuge goes faster than the regular centrifuges that allow 50mL tubes
- Flow Cytometry:
in vitro pathway assays (CO2 project)
- Cell Prep (in progress)
- Crude Extract Prep (in progress)
- Sample Analysis via UPLC-MS/MS (in progress)
