Lidstrom:Assaying enzyme libraries

From OpenWetWare
Jump to navigationJump to search

Back to Protocols

Guiding Principles

  • What range of detection do you want relative to your + control?
    • If you are looking for high activity mutants, make sure you aren't saturating the linear range of your detection capabilities.
    • If you are looking for lower activity mutants, make sure your lower limit of detection is suitable.