Biomod/2011/TUM/TNT/LabbookA/2011/10/14: Difference between revisions

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according to following scheme: <br>
according to following scheme: <br>
http://openwetware.org/wiki/Image:Pipet_plan_D4-D5.xls <br>
http://openwetware.org/wiki/Image:Pipet_plan_D4-D5.xls <br>
due to the concentration problems, a new purification of BM2 was used; dilution of the structure was again 1:25 <br>
D4: BM2 with one DAPI molecule in average every 7bp <br>
D4: BM2 with one DAPI molecule in average every 7bp <br>
D5: BM2 with one DAPI molecule in average every 21bp <br>
D5: BM2 with one DAPI molecule in average every 21bp <br>

Revision as of 04:10, 14 October 2011

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TEM

prepared new grids with DAPI

according to following scheme:
http://openwetware.org/wiki/Image:Pipet_plan_D4-D5.xls
due to the concentration problems, a new purification of BM2 was used; dilution of the structure was again 1:25
D4: BM2 with one DAPI molecule in average every 7bp
D5: BM2 with one DAPI molecule in average every 21bp