Biomod/2011/TUM/TNT/LabbookA/2011/10/14

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14th Oct 2011

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TEM

Prepared new Grids with DAPI

According to following scheme:
http://openwetware.org/wiki/Image:Pipet_plan_D4-D5.xls
Due to the concentration problems, a new purification of BM2 was used; Dilution of the structure was 1:25.
D4: BM2 with one DAPI molecule in average every 7bp
D5: BM2 with one DAPI molecule in average every 21bp

Observed TEM Grids

2C1: BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp): good stain but slightly to low structure concentration, 32 images 2C2: BM2 1:40 with 0.69 µM ethidium bromide (corresponding to one molecule every 21bp): good stain but slightly to low structure concentration, 31 images 2B4: BM2 1:40 with 2.27 µM ethidium bromide (corresponding to one molecule every 7bp): good stain but slightly to low structure concentration, 35 images