Biomod/2011/TUM/TNT/LabbookA/2011/10/13

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13th Oct 2011

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Contents

Single Molecule Fluorescence Microscopy Measurement of BM21_172/173 with/without Spermine

Aim Seeing tendencies in changes in length of the origami when the intercalator spermine is added.

Preparation

For calculations see Calculation of intercalator concentrations.

Measurement

  • The excitation time was set to 0.1s/frame.
  • 500 Pictures per video were taken.

Results

The amount of FRET events found in Matlab analysis was too low for a reliable interpretation.

Observed TEM Grids

  • 2B3 2.27µM EtBr: good stain and dilution