User talk:Brian P. Josey/Notebook/2009/08/28
Steve Koch 23:37, 28 August 2009 (EDT): Thanks, Ant, for teaching Brian! Here's my take (you can develop your own technique in time):
- For the same sized gel, I used 25 ml
- 0.8% is probably better for the longer fragments that we're typically looking at
- I didn't add back missing water. Not sure, though, if this is more of a problem in ABQ than Ithaca
Brian Josey 10:37, 28 August 2009 (EDT): Thanks for the advice. Anthony said that I do not have to replace the water that evaporates off, but I wrote down that step just in case it ever became a problem.