User talk:Anthony Salvagno/Notebook/Research/2010/06/22/Tethering Planning Notes
Steve Koch 15:08, 23 June 2010 (EDT): I searched my old notes and actually couldn't find anything showing a dramatic shift of a long template from neutravidin binding. So, I don't know whether it's possible to check your final template. However, I do have a document showing it's easy to see shift on oligos and hyrbridized oligos. So you could check the duplex. I put the document in my public dropbox, I think this link will work: http://dl.dropbox.com/u/8202917/001116_ShiftingGel_ForksAndOligos.doc I notice that I was worried about protein unbinding so I ran the gel in the fridge. I'm not sure, though, that's necessary. My gut right now tells me that you shouldn't worry about shifting the long template. But it may be worth making sure the single oligo is biotinylated.