User talk:Anthony Salvagno/Notebook/Research/2009/07/15/PCR cleanup and Gel of 4.4kb tethers
Steve Koch 13:04, 16 July 2009 (EDT): I was right that you don't need the sodium acetate. Buffer PB doesn't even have the pH indicator. I don't know why they changed their instructions like that--sorta weird. In any case: it worked like crazy! Congrats! The only thing I can't say is whether the DNA seems uniform in length, because I didn't dilute it. In the fridge, in the white rack w/ beads, there's a 1:10 dilution of reaction #1, with about 90 ul left. You could dilute this 1:10 and do another tethering assay and my bet is that it will work perfectly. E.g. 1.5 ul in 15 ul BGB that you use for the incubation step.