User:Zachary I. Mendel/Notebook/Zacks Notebook/2013/11/19

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Project name Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

What was done today

Today we made Au-NPs with the protein's invertase and BSA

Procedure

New Nanoparticle Synthesis: Please choose two from the following sets!

  1. Bovine serum albumin
    1. Ratios
      1. 105
      2. 110
      3. 115
      4. 120
      5. 125
      6. 130
      7. 135
      8. 140
      9. 145
      10. 150
  2. Catalase
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
  3. Invertase
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210
  4. Elastin
    1. Ratios
      1. 30
      2. 50
      3. 70
      4. 90
      5. 110
      6. 130
      7. 150
      8. 170
      9. 190
      10. 210

Notes

Table 1. Preparation of Gold Standards

Concentration(μg/mL) Volume of stock Au solution(mL) Volume Deionized water added(mL)
10 0.1 9.9
20 0.2 9.8
30 0.3 9.7
40 0.4 9.6
50 0.5 9.5


Table 2. Preparation of BSA stock solution

Preparation of BSA stock solution '
Molecular weight (g/mol) 66000
mass (g) 0.0432
volume stock (L) 0.03
Concentration (M) 2.18182E-05


Table 3. Preparation of Invertase Stock solution

Preparation Invertase Stock '
Molecular weight (g/mol) 135000
mass invertase (g) 0.1015
volume stock (L) 0.025
concentration (M) 3.00741E-05


Table 4. Preparation of BSA-AuNP solutions

Ratio Amount of gold added(mL) concentration BSA (M) volume BSA (mL) volume Water added (mL)
105 0.347660664 2.38095E-06 0.545634921 4.106704416
110 0.347660664 2.27273E-06 0.520833333 4.131506003
115 0.347660664 2.17391E-06 0.498188406 4.15415093
120 0.347660664 2.08333E-06 0.477430556 4.174908781
125 0.347660664 0.000002 0.458333333 4.194006003
130 0.347660664 1.92308E-06 0.440705128 4.211634208
135 0.347660664 1.85185E-06 0.424382716 4.22795662
140 0.347660664 1.78571E-06 0.40922619 4.243113146
145 0.347660664 1.72414E-06 0.395114943 4.257224394
150 0.347660664 1.66667E-06 0.381944444 4.270394892


Table 5. Preparation of Invertase-AuNP solutions

Ratio Amount gold added (mL) Concentration Invertase (M) Volume Invertase (mL) Volume water added (mL)
30 0.347660664 8.33333E-06 1.38546798 3.266871356
50 0.347660664 0.000005 0.831280788 3.821058548
70 0.347660664 3.57143E-06 0.593771992 4.058567345
90 0.347660664 2.77778E-06 0.46182266 4.190516676
110 0.347660664 2.27273E-06 0.377854904 4.274484432
130 0.347660664 1.92308E-06 0.31972338 4.332615956
150 0.347660664 1.66667E-06 0.277093596 4.37524574
170 0.347660664 1.47059E-06 0.244494349 4.407844987
190 0.347660664 1.31579E-06 0.218758102 4.433581234
210 0.347660664 1.19048E-06 0.197923997 4.454415339