User:Trisha I. Ibeh/Notebook/Trisha Notebook/2013/10/30

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Objective

  • Redo Atomic Absorption from the previous lab (10/29/13).
  • Complete UV Vis for the proteins made in the previous week (Myoglobin-AuNPs and HRP-AuNPs).

Procedure

  1. Atomic Absorption
    1. centrifuge (4mL of your) samples (use the large centrifuge upstairs) 30min at 4000rpm.
    2. make standard solutions in the same manner as the last time we performed AA. But, this time make concentrations that go up to 50ug/mL
    3. Take measurements of your standards and samples
    4. Make a graph showing concentration of Au vs Au:protein ratio
  2. UV Vis
    1. centrifuge 1mL of your sample 30min at max rpm
    2. Take measurements of your samples

Data

Graphs of the UV Vis of the Myoglobin- AU NPs and the HRP AU-NPs. The UV VIS conducted on the HRP- AuNP samples follow a clear and concise trend as the AU: HRP ratio increased.

  • Screen Shot 2013-11-03 at 3.09.23 AM.png
  1. Series 1: 230
  2. Series 2: 250
  3. Series 3: 270
  4. Series 4: 290
  5. Series 5: 310
  6. Series 6: 330
  7. Series 7: 350
  8. Series 8: 370
  9. Series 9: 390
  10. Series 10: 410


The Myoglobin One can hypothesize that if the Au:Myoglobin increased like in the case of HRP, the trend will be more easily observed.

Screen Shot 2013-11-03 at 3.20.14 AM.png