User:Tara K. Luckau/Notebook/Team ConGen/2013/02/09

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ASHY Cvanµ23

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Gela.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ24

PCR

  • 20130209 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Gelb.png

  • it works! Yay! Many possibilities


ASHY Cvanµ26

PCR

  • 20130209 PCRb.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Gelc.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ27

PCR

  • 20130209 PCRc.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130209 Geld.png

  • mispriming everywhere - primer pair not usable for ASHY