User:Tara K. Luckau/Notebook/Team ConGen/2013/02/08

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ASHY Cvanµ18

Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130208 Gela.png

  • mispriming everywhere - primer pair not usable for ASHY


ASHY Cvanµ19

PCR

  • 20130208 PCRa.png


Gel

Pour Load Run
270 mL 1x TAE + 5.4 g agarose 2 µL gel load dye + 4 µL PCR product 160 V
27 µL GelRed 6 µL ladder 45 minutes


20130208 Gelb.png

  • no amplification - primer pair not usable for ASHY


ASHY Cvanµ23

PCR

  • 20130208 PCRb.png