User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2012/04/12

From OpenWetWare
Jump to: navigation, search
BDLlogo notext lr.png AU Biomaterials Design Lab Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png


Objective

Begin growth of BL21(DE3) E.coli with mutated GFP plasmid (D1C) for expression.

Description

  1. LB was kindly prepared by Dr. Hartings for this expression.
  2. 25μL of 100mg/mL ampicillin was added to 25mL of LB (four of these).
  3. One colony was added to each of these flasks.
  4. The flasks were incubated overnight at 37°C at 250rpm.

Notes

There was no growth overnight. The plate that the colonies came from was probably too old, and also may have been contaminated.