User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2012/04/12

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Begin growth of BL21(DE3) E.coli with mutated GFP plasmid (D1C) for expression.


  1. LB was kindly prepared by Dr. Hartings for this expression.
  2. 25μL of 100mg/mL ampicillin was added to 25mL of LB (four of these).
  3. One colony was added to each of these flasks.
  4. The flasks were incubated overnight at 37°C at 250rpm.


There was no growth overnight. The plate that the colonies came from was probably too old, and also may have been contaminated.