User:Tamra L. Fisher/Notebook/Experimental Biological Chem/2012/04/11
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Try to extract the GFP from the dialysis buffer. Make MBP-intein gold nanoparticles.
Extracting GFP Protein
Today we tried to extract our GFP protein from the dialysis buffer it had leaked into yesterday. We were not able to concentrate it, or get it to stick to a nickel column. Because of this we will need to re-express and purify the GFP again over the weekend.
MBP-intein Gold Nanoparticles
When removed from the oven the MBP-intein/gold solution was a light purple color.