User:Tamanika Tinsley/Notebook/Chem 581 Biomaterials Design Lab/2014/10/08

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Oct 8 - Fluorescence Group Members Andrew Farag Sr (Undergrad) and Michael Bible Jr (Undergrad) Group Name : AMT Objective To make stock solutions To take UV-Vis and Fluorescence spectra of prepared R6G samples To make a calibration curve using both fluorescence and UV-Vis data To finish film synthesis Notes The completion of the film synthesis used the procedure from Tami's Notebook R6G Fluorescence: Calibration and Measurement Completed by Michael Make stock concentrations (both groups can use the same solutions) Initial stock: 2500μM, secondary stock: 500μM 0.10μM Made with 500μM solution 0.50μM Made with 500μM solution 1.0μM Made with 500uM solution 1.5uM Made with 500μM solution 2.0μM Made with 2500μM solution 0.75μM Made with 500μM solution 1.2μM Made with 500μM solution Thanks to Madeleine for making initial 2500μM solution of R6G. Fluorimeter Settings 500nm excitation 515-700nm scan range 10.0nm slit width 200nm/min scan speed