User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/31

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Testing Gold and Protein in Small Hydrogels

  • Using two identical sets of hydrogels - 0.1g:1mL H2O; 0.15g:1mL H2O; 0.2g:1mL H2O each for PVA 31,000, PVA 89-98,000, and PVA 130,000.
    • 1 set of hydrogels were put into a centrifuge at 10:00AM to pull the water out, then given 0.25mL Au and 0.25mL BSA.
    • The other set were given 0.25mL Au and 0.25mL BSA after pouring out excess water.
  • Both were put into the oven at 11:30AM.

Examining Hydrogels Infused with BSA&Au

  • 15mL hydrogels were put into the oven at approximately 11:30AM.

Making New Small Hydrogels

  • Make another 2 sets of mini hydrogels for future use.
    • 0.1g:1mL H2O; 0.15g:1mL H2O; 0.2g:1mL H2O each for PVA 31,000, PVA 89-98,000, and PVA 130,000.
      • Actual values:
        • PVA 31-50,000
          • .1000g, .1001g, .1500g, .1500g, .2002g, .2000g (all still given 1mL of water)
        • PVA 89-98,000
          • .1001g, .1000g, .1500g, .1500g, .2000g, .2001g
        • PVA 130,000
          • .0999g, .1000g, .1499g, .1502g, .2001g, .2001g
    • One set put into water bath at 80°C at 12:30PM, then taken out and put into sonicator at 1:30PM. Vials were put in freezer at 2:30PM after the solution was homogeneous.
    • The second set was put into the water water bath at 80°C at 1:30PM, then taken out and put into sonicator at 2:30PM. Vials were put in freezer at 3:30PM after the solution was homogeneous.