User:Stephanie Tietz/Notebook/Stephanies Notebook/2013/05/24

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Making Small Hydrogels

  • PVA 31,000
    • 2 sets of 0.1g:1mL H2O; 0.15g:1mL H2O; 0.2g:1mL H2O
    • Put into water bath at 80°C at 12:30PM, then taken out and put into sonicator at 1:30PM. Vials were put in freezer at 2PM after the solution was homogeneous.
  • PVA 89,000-98,000
    • 2 sets of 0.1g:1mL H2O; 0.15g:1mL H2O; 0.2g:1mL H2O
    • Put into water bath at 80°C at 1:30PM, then taken out and put into sonicator at 2:30PM. Vials were put in freezer at 3:40PM after the solutions were homogeneous.
  • PVA 130,000
    • 2 sets of 0.1g:1mL H2O; 0.15g:1mL H2O; 0.2g:1mL H2O
    • put into water bath at 80°C at 2:40PM, then taken out and put into sonicator at 3:40PM. Vials were put in freezer at 4:40PM after the solutions were homogeneous.

Nanoparticle Formation in Already Formed Hydrogels

  • 30mL hydrogels with PVA 31,000, 89-98,000, and 130,000 molecular masses (2g:1mL ratio) were cut into two slices, making each slice approximately 15mL hydrogels.
  • One slice was squeezed of excess water.
  • All of the slices were given 1.5mL Au, 1.5mL BSA, and 12mL H2O and then were put into the freezer.