User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/05/02

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Incorporation of PCR BB product into backbone (2nd attempt)

Cut PCR results and three different backbones (amp+kan, amp+cm, amp+tet) seperately with EcoRI and PstI, then denatured restriction enzymes. Ligated DNA together with T4 DNA ligase.
Knight:DNA_ligation_using_T4_DNA_ligase
Transformed ligated product into E.coli by heatshock
Transforming_chemically_competent_cells_(Inoue)
Plated transformants onto respective plate (amp+kan, amp+cm, amp+tet).