User:Spenser Skates/Notebook/pTKX luciferase biobrick/2008/04/23

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Turned operon into biobrick part with PCR

Used PCR to turn the sequence containing the ORF's lux A-E into a biobrick part. Used the following primers:

GTTTCTTCGAATTCGCGGCCGCTTCTAGAGAGGAGgcaaatatgactaaaaaaatttcattcattattaacg,luxF
GTTTCTTCTACTAGTAGCGGCCGCTGCAGtcaactatcaaacgcttcggttaag,luxR