User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/15

From OpenWetWare
Jump to navigationJump to search
Project name Main project page
Previous entry      Next entry

Ocean Optics

Purpose

To use ocean optics to make conclusions about the rate of myoglobin templated Au nanoparticle formation in pH 10

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 2.96mM
      1. (0.25mM)(3.0mL)=(XmL)(2.96mM)
      2. XmL= .253mL= 253uL Au
    2. Myoglobin 141.2uM
      1. (12.5uM)(3.0mL)=(XmL)(141.2uM)
      2. X=0.265mL= 265uL Myoglobin
    3. 300uL 1mM NaOH
    4. Water
      1. Volume of water= 3000uL- 253uL-265uL-300uL= 2182uL
    5. Scans were taken at 80oC every 2 minutes for 3 hours

Data

Absorbance pH10

Wavelength pH10


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Sara_K._Mattson/Notebook/Chemistry_671/2016/11/15&oldid=1018795"