User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/16

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Fluorescence Purpose Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 10 Procedure Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total Au 2.96mM (0.25mM)(3.0mL)=(XmL)(2.96mM) XmL= .253mL= 253uL Au Myoglobin 141.2uM (12.5uM)(3.0mL)=(XmL)(141.2uM) X=0.265mL= 265uL Myoglobin 300uL 1mM HCl Water Volume of water= 3000uL- 253uL-265uL-300uL= 2182uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm Data AuNP pH10 scans Wavelength of Myoglobin AuNP's at pH 10 Integrated intensity of Myoglobin AuNP's at pH 10