User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/08

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Fluorescence Purpose Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 9 Procedure Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total Au 3.45mM (0.25mM)(3.0mL)=(XmL)(3.45mM) XmL= .217mL= 217uL Au Myoglobin 133uM (12.5uM)(3.0mL)=(XmL)(141.2uM) X=0.266mL= 266uL Myoglobin 30uL 1mM NaOH Water Volume of water= 3000uL- 217uL-266uL-30uL= 2467uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm Data Scans AuNP pH 9 Wavelength of Myoglobin AuNp's at pH 9 Maximum Intensity of Myoglobin AuNp's at pH 9 Integrated Intensity of Myoglobins AuNp's at pH 9 Solution Preparation for UV-VIS Stock Concentrations: Myoglobin 141.7mM Silver 34.3mM Fructose 53.1mM All concentrations of fructose were redone at pH11 All concentrations of fructose were redone at pH12 pH 5 and 9 were repeated for 0mM Fructose pH 5 and 9 were repeated for 0.0625mM Fructose Note: for pH 9 for both concentrations of fructose, 1mM NaOH was used, NOT 1M NaOH