User:Sara K. Mattson/Notebook/Chemistry 671/2016/11/03

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Fluorescence Purpose Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH 5 Procedure Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total Au 2.64mM (0.25mM)(3.0mL)=(XmL)(2.64mM) XmL= .284mL= 284uL Au Myoglobin 103uM (12.5uM)(3.0mL)=(XmL)(103uM) X=0.364mL= 364uL Myoglobin 30uL 1mM HCl Water Volume of water= 3000uL- 284uL-364uL-30uL= 2302uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm Data Scans AuNP pH 5 Wavelength of Myoglobin AuNp's at pH 5 Maximum Intensity of Myoglobin AuNp's at pH 5 Integrated Intensity of Myoglobins AuNp's at pH 5