User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/04

From OpenWetWare
Jump to navigationJump to search
Project name Main project page
Previous entry      Next entry

Ocean Optics


To use ocean optics to make conclusions about the rate of nanoparticle formation in pH6.


  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, .001mM HCl 3mL total
    1. Au 2.26mM
      1. (0.25mM)(3.0mL)=(XmL)(2.26mM)
      2. XmL= .332mL= 332uL Au
    2. Myoglobin 160uM
      1. (12.5uM)(3.0mL)=(XmL)(160uM)
      2. X=0.234mL= 234uL Myoglobin
    3. HCl 0.001mM
      1. (3.0mL)(0.001mM)=(1.0mM)(XmL)
      2. XmL=0.003mL= 3uL HCl
    4. Water
      1. Volume of water= 3000uL- 332uL-234uL-3uL= 2431uL
    5. Scans were taken at 80oC every 2 minutes for 3 hours
  2. Scans

Absorbance of Myoglobin Au NP's at pH5

Wavelength of Myoglobin Au NP's at pH5