User:Sara K. Mattson/Notebook/Chemistry 671/2016/10/05

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Fluorescence Purpose Use fluorescence to examine the rate at which Myoglobin templated gold nanoparticles unfold at pH6 Procedure Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, .001mM HCl 3mL total Au 2.26mM (0.25mM)(3.0mL)=(XmL)(2.26mM) XmL= .332mL= 332uL Au Myoglobin 160uM (12.5uM)(3.0mL)=(XmL)(160uM) X=0.234mL= 234uL Myoglobin HCl 0.001mM (3.0mL)(0.001mM)=(1.0mM)(XmL) XmL=0.003mL= 3uL HCl Water Volume of water= 3000uL- 332uL-234uL-3uL= 2431uL Absorbance measurements were taken and saved every 3 minutes for 3 hours The instrument was set at 80 degrees Celsius The scan range parameters were start at 310nm and ending at 540nm The emission and exit slit were set at 10nm Graphs Wavelength of Myoglobin AuNp's at pH6 Maximum Intensity of Myoglobin AuNp's at pH6 Integrated Intensity of Myoglobins AuNp's at pH6 Solution for UV-Vis Preparation Stock Concentrations: Myoglobin 190uM Silver 16.6mM Fructose 40.9mM 0 mM Fructose pH μL of 1 mM HNO3stock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AgNO33stock μL of Fructosestock μL of Myoglobinstock μL of Water 4 500 0 0 75 0 329 4096 5 50 0 0 75 0 329 4546 6 5 0 0 75 0 329 4591 7 0 0 0 75 0 329 4596 8 0 5 0 75 0 329 4591 9 0 50 0 75 0 329 4546 10 0 500 0 75 0 329 4091 11 0 0 5 75 0 329 4591 12 0 0 50 75 0 329 4546 0.0625 mM Fructose pH μL of 1 mM HNO3stock μL of 1 mM NaOHstock μL of 1 M NaOHstock μL of AgNO33stock μL of Fructosestock μL of Myoglobinstock μL of Water 4 500 0 0 75 8 329 4088 5 50 0 0 75 8 329 4538 6 5 0 0 75 8 329 4583 7 0 0 0 75 8 329 4588 8 0 5 0 75 8 329 4583 9 0 50 0 75 8 329 4538 10 0 500 0 75 8 329 4088 11 0 0 5 75 8 329 4583 12 0 0 50 75 8 329 4538 Notes The silver nitrate needed to be handled in a dark room, and the test tubes were completely wrapped in tin foil because it is a light sensitive solution.