User:Roberta Diaz Jimenez/Notebook/CHEM 472/2016/02/17

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Objectives Set up 1ml AgNP and AuNP synthesis reactions for SPI, WPI, Casein, Pea Protein and Brown Rice Protein Set up 20ml Pea Protein AgNP and Brown Rice Protein AgNP reactions Analyze Reactions from 02/16/16 with UV Vis Materials Used same nutritional proteins on 02/02/16 Protein and gold stocks prepared fresh every day Ocean Optics UV Vis was used to record UVVIS Spectra of Casein AgNPs. The other machine broke and is unusable. Integration time= 10ms and Average =100. A water blank in the same quartz cuvette was subtracted from the spectra before running any samples. Procedure Further reduce stock protein concentrations (and thus concentrations in reaction mix) to increase metal/protein ratio to spur NP formation as we saw protein aggregate and nanofiber formation in previous reactions increase concentration from 02/16/16 trial comparing citrate to no citrate samples, and vary citrate concentration Prepare 20ml reaction to synthesize large quantities of reactions known to work Pea Protein and Brown Rice Protein AgNP Note that spectra of reaction mixes before reacting need to be taken Also note that this is just one size of nanoparticle that can be made with these reactions, its possible to change variables and change size and functionality of nanoparticle, these were just the first reactions to work. Detailed Reaction Setup shown Below (4 hours 80˚C, 1ml rxns) 20 mL Reactions ' ' ' ' ' ' ' ' Vial # 2.5mM HAuCl4 (µL) 5 mMAgNO3 (µL) Protein (µL) 10 mM Citrate (µL) Water (µL) Worked? Notes Casein 1 ? 0 ? 5 mg/mL 2 0 800 11200 0 8000 ✓ Run UV/Vis Soy 3 800 0 4000 400 14800 ✓ Run UV/Vis 0.25 mg/mL 4 0 ? ? 0 Whey 5 800 0 4000 400 14800 ✓ Run UV/Vis 0.25 mg/mL 6 0 ? ? 0 Pea 7 ? 0 ? 0.15 mg/mL 8 0 800 4000 0 15200 Redo Rice 9 ? 0 ? 0.15 mg/mL 10 0 800 4000 0 15200 1mL Reactions Vial # 5.0 mM HAuCl4 (µL) 5.0 mMAgNO3 (µL) Protein (µL) 10 mM Citrate (µL) Water (µL) Worked? Notes 1 40 0 200 0 760 0.25 mg/mL Casein 2 40 0 200 20 740 Stock: 15mL 3 40 0 200 40 720 4 40 0 200 60 700 5 0 40 200 0 760 6 0 40 200 80 680 0.25 mg/mL Soy 7 0 40 200 100 660 Stock: 10mL 8 0 40 200 150 610 9 0 40 200 200 560 10 0 40 200 0 760 11 0 40 200 80 680 0.25 mg/mL Whey 12 0 40 200 100 660 Stock: 10mL 13 0 40 200 150 610 14 0 40 200 200 560 15 40 0 200 0 760 0.15 mg/mL Pea 16 40 0 200 20 740 Stock: 22 mL 17 40 0 200 40 720 5.48g 18 40 0 200 60 700 19 40 0 200 0 760 0.15 mg/mL Rice 20 40 0 200 20 740 Stock: 27mL 21 40 0 200 40 720 6.75g 22 40 0 200 60 700 Results 20ml Samples Brown Rice Protein AgNP solution turned gold after reaction, even before reaction started after 10 minutes at room temperature started to form AgNPs and turned slightly yellow Pea Protein solution remained clear with some white precipitation, possibly not total dilution Detailed results, data and procedure: SRB Lab Notebook Data for this entry's date