User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/10/06

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Spermatogenesis

  • Wash
    • Wash all 3 sets 3 x 15' in PBST
  • Secondary Antibody (1:500 in 2% NGS)
    • Tdt-Myb anti-mRFP incubate with anti-rabbit 488 O/N at 4°C
    • GFP-Myb anti-BAM incubate with anti-mouse 633 O/N at 4°C
    • GFP-Myb anti-SA incubate with anti-guniea pig 633 O/N at 4°C