User:Robert J. Schiemann/Notebook/Lipsick Lab/2009/10/05

From OpenWetWare
Jump to: navigation, search
Owwnotebook icon.png Drosophila melanogaster Genetics Report.pngMain project page
Resultset previous.pngPrevious entry      Next entryResultset next.png

Spermatogenesis

  • Dissect 3 sets of testis
    • 8 from Tdt-Myb
    • 2 x 8 from GFP-Myb, H2Av-RFP
  • Fix
    • 30' in 4% Formaldehyde at RT
    • Wash 4 x 5' at RT
  • Block
    • 1 hr at RT in 2% NGS
  • Primary Antibody
    • Tdt-Myb with 1:1000 anti-mRFP O/N at 4°C
    • GFP-Myb with 1:5 anti-BAM O/N at 4°C
    • GFP-Myb with 1:100 anti-SA O/N at 4°C