User:Rebeca Rodriguez/Notebook/Chem 471/2015/11/03

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Objective

Ocean Optics Kinetics on degradation of AuNP Fibers by 100 nM proteinase K.

Protocol

5 1 mL AuNP fiber samples were centrifuged (and supernatant fluid removed), and combined into one 1 mL sample in Tris/NaCl buffer solution. A small volume of proteinase K was added such that the the volume of proteinase K in the cuvette was 100 nM. The degradation of the AuNP fibers was then monitored using the Ocean Optics UV-Vis. Spectra were taken every 2 minutes, with one spectrum taken before the addition of the proteinase K.

Set up protocols for the Ocean Optics UV-Vis can be found here.

Data

Ocean Optics Spectra .png

Spectra for the first 60 minutes of the degradation of AuNP fibers by proteinase K.

Ocean Optics Kinetics Nov 3.png

Peak absorbance values from the spectra in the first spectra plotted against time.

Ocean Optics Initial 6 min Nov 3.png

Slope of the first six minutes of the kinetics shown in the second figure. From this graph, it is possible to determine Vinitial for the degradation of AuNP Fibers by 100 nm Proteinase K.

Note

Michael was wonderful enough to analyze this data while I did Bradford/freaked out about my life. He deserves all of the subway sandwiches in the world.