UV-Vis standards
- Standards needed to be made of R6G in order to calculate the amount of dye that was absorbed and how much dye remains in the solution.
Molar Absorptivity of R6G: 116000 LMol-1cm-1
Assumed stock concentration: .005 M
- Because the absorbance of R6G is so high, standard concentrations needed to be diluted to below 8 μM.
Table 1. Standard concentrations in 2mL solutions
Standard Concentrations (uM)
|
Actual Concentration (M)
|
Absorbance
|
0 |
0 |
0
|
2 |
1.59483E-06 |
0.185
|
4 |
3.18966E-06 |
0.37
|
6 |
4.78448E-06 |
0.555
|
8 |
6.37931E-06 |
0.74
|
- The concentration of the original stock solution was 398.7μM
Table 2: Actual concentration of dye in eppendorf samples
Sample number
|
believed concentration
|
Concentration in 1mL solution (uM)
|
molar concentration in epp tubes (M)
|
1 |
20 |
15.94827586 |
1.59483E-05
|
2 |
40 |
31.89655172 |
3.18966E-05
|
3 |
60 |
47.84482759 |
4.78448E-05
|
4 |
80 |
63.79310345 |
6.37931E-05
|
5 |
100 |
79.74137931 |
7.97414E-05
|
6 |
150 |
119.612069 |
0.000119612
|
7 |
200 |
159.4827586 |
0.000159483
|
Notes
-These samples were left for too long without being tested and resulted in some of the dye releasing out of the complexes. For this reason, UV-Vis of these samples were not fully run and new samples were made.
|