Dye Addition
- Preparation of Rhodamine 6G stock solution
| Rhodamine 6G
|
'
|
| Molecular Weight (g/mol) |
479.02
|
| mass(g) |
2.3951
|
| volume stock (L) |
0.01
|
| Concentration (M) |
0.5
|
| Real concentration of Rhodamine used
|
'
|
| concentration (M) |
0.005
|
| volume stock (L) |
0.1
|
Dye addition to samples was done at a range of concentrations from 20 μM - 200 μM and was added to 1mL of sample. The samples need at least 24 hours in order to soak in the dye.
- Fluorescence of the water sample which the nanoparticles/ rhodamine will be analyzed to determine the total R6G concentration adsorbed by the hydrogel complexes.
| Sample Number
|
Internal Conc. Dye in each sample (M)
|
volume of dye added
|
in uL
|
| 1 |
0.00002 |
0.000004 |
4
|
| 2 |
0.00004 |
0.000008 |
8
|
| 3 |
0.00006 |
0.000012 |
12
|
| 4 |
0.00008 |
0.000016 |
16
|
| 5 |
0.0001 |
0.00002 |
20
|
| 6 |
0.00015 |
0.00003 |
30
|
| 7 |
0.0002 |
0.00004 |
40
|
Samples were placed in eppendorf tubes and manually shaken/ stirred for approximately 2 minutes each in order to mix dye and prepare samples for absorption/ crosslinking. Samples were left overnight.
NOTES
- From UV-Vis spectra collections, it was determined that the true concentration of the R6G that was used was: 398.7 uM
| 
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