User:Pauline Wonnenberg/Notebook/CHEM 471 Fall 2017/2017/09/13

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Objectives Prepare 50mL of stock solution of BSA that is roughly 5mg into 5ml of saline Calculate your actual solution concentration Record UV-VIS spectra between 200nm and 800nm Make 8 standard solutions (1mL each) between 1µg/mL and 20µg/mL Record UV-VIS spectra of the above prepared solutions Make duplicate blanks (4 solutions total) Description Prepare 50mL of stock solution of BSA 5mg into 5ml of saline Calculate your actual solution concentration (5E-3grams)/(66,000g/mol)=7.58E-8mol of BSA (7.58E-8mol of BSA)/(0.005L)=1.52E-5M Make 8 standard solutions (1mL each) between 1µg/mL and 20µg/mL Make duplicate blanks (4 solutions total) Data Record UV-VIS spectra between 200nm and 800nm UV-VIS results from the 8 standard solutions prepared Zoom on the peak obtained from UV-VIS Record UV-VIS spectra of the blank prepared solutions UV-VIS of 1mL Tris/NaCl Buffer UV-VIS of 200µL Bradford reagent + 800µL buffer