User:Pauline Wonnenberg/Notebook/CHEM 471 Fall 2017/2017/09/12

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Objective Prepare 0.1M buffer solutions of pH 10, 11, & 12 Make a 10mM HAuCl4 solution and a 100mM AA solution (using an AA with a basic R group) Prepare Buffer for Bradford Analysis Prepare Buffer for Dialysis Prepare Buffer for SDS-PAGE Description Prepare 0.1M bugger solutions of pH 10, 11, & 12 Following the previous AA mass calculations. Buffers at pH 10, 11, & 12 were used instead of water. Make a 10mM HAuCl4 solution and a 100mM AA Solution (using AA with a basic R group) 10mM calculation HAuCl4 (339.785g/mol) solution (10E-3M)*(0.25L)=0.0025moles (0.0025moles)(339.785g/mol)=0.8495 grams 100mM AA solution (Arginine = 174.2g/mol) (100E-3M)*(0.04LL)=0.004moles (0.004moles)(174.2g/mol)=0.70grams Prepare Buffer for Bradford analysis 1L of 50mM Tris, 50mM NaCl pH7.5 Tris (121.14g/mol) mass calculations (1L)*(50E-3M)=0.05mol (0.05mol)*(121.14g/mol)=6.057grams NaCl (58.44g/mol) mass calculations (1L)*(50E-3)=0.05mol (0.05mol)(58.44g/mol)=2.922grams pH measurements in order pH=10.34; 9.55; 9.08; 8.53; 8.11; 7.77; 7.47 Prepare buffer for Dialysis Prepared on September 19th, 2017 Prepare Buffer for SDS-PAGE Prepared on 9September 26th, 2017 Data There was no data to report from these solution preparations.