User:Paul Rothenberg/Notebook/Pauls Notebook/2014/03/05

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Heme Extraction from Myoglobin Preparation of Apoprotein: 24.3 mg of Tris was added to 4 mL of water to make a 0.05M solution to dissolve 200 mg of myoglobin 3.36 g of Sodium Bicarbonate was dissolved in 4L of H2O to make a 10 mM dialysis buffer The pH of the Tris solution was brought down to 2.3-2.5 at 0°C before 2-butanone extraction was performed 2-butanone was placed on ice for the extraction Acid/2-butanone extraction was performed on the protein solution with equal parts 2-butanone and protein The aqueous extract was then added to a dialysis cassette and placed in the dialysis buffer in the 0°C cold room and will sit overnight Approximately 3 mL of aqueous extract was extracted overall