User:Paul Rothenberg/Notebook/CHEM 571 Fall 2014/2015/03/31
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New Reaction/Sampling Protocol
Here's the new protocol we developed for analysis:
Notes from Protocol Prep
When trying to resuspend the fibers in solution, they were vortexed gently. The solution became colloidal (purple) in color with fibers suspended. When the supernatant was extracted out and replaced with buffer, the fibers did not reconstitute into solution as expected. The solution was sonicated; it appeared that the sonication possibly created a colloidal solution from the fibers.
Instead, the fibers will be dispersed in the individual test tubes, and concentrated buffer added. This will prevent excess transferring of fibers and dispersion of fibers into colloid.
The new protocol should be something similar to this:
This should minimize the errors from the protocol we ran today.