User:Patrick Hampson/Notebook/chem471/2016/10/26

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BSA Ocean Optics pH9

Final Solution Concentrations

  1. pH 9
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 52.8 uM
  2. [Au]= 2.64 mM
  3. [NaOH]= 1 mM

Volumes for making the solution:

  1. 52.8 uL BSA
  2. 284.09 uL Au
  3. 177.6 uL H2O
  4. 30 uL NaOH


The graph above displays the absorbance over time for our pH 9 solution. There is a clear increase in absorbance over time. As previously seen, the beginning (0-4000 seconds) is exponential. The middle (4000-8000 seconds) is linear. The end (8000-~11000 seconds) is logrithmic.

The graph above displays the maximum wavelength over time. There is a consistent maximum of 486/487 until about 3800 seconds (the point we observed a spike in pH8). The maximum then spikes to 550 (as we have seen in pH8). It immediately drops down to approximately 535nm. The maximum then fluctuates between 532 and 540 nm for the remainder of the experiment.

Th graph above displays the absorbance at wavelength 535.15nm versus time. As we have previously seen in every other parallel comparison, the graph mimics the general absorbance graph.