User:Patrick Hampson/Notebook/chem471/2016/10/19

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  • BSA pH8

Sample Preparation

BSA Fluorescence Solution

  1. pH 8
  2. [Au]=0.25 mM
  3. [BSA]=3.125 uM
  4. Vf=3000 uL

Stock Concentrations 160920

  1. [BSA]= 63.1 uM
  2. [Au]= 2.39 mM
  3. [NaOH]= 1 mM

Volumes for making the solution:

  1. 148.9 uL BSA
  2. 255.6 uL Au
  3. 2565.4 uL H2O
  4. 3 uL NaOH


BSA Fluor pH8 emissionsmax.PNG

The graph above displays the flourescence emission intensity over time. There is an initial decrease at the beginning, where a plateau is held just above 300nm until approximately 3000 seconds. The emission intensity then jumps to approximately 420-425 nm, fluctuation between the two for the remainder of the run.

BSA Fluor pH8 integratedemissions.PNG

The graph above displays integrated intensity over time. The intensity increases sharply from 2000 to approximately 5100 at 1800 seconds. From its peak it declines steadily until about 4000 seconds, where it is a value of about 4400. After 4000 seconds, there is a consistent minor increase in intensity for the duration of the experiment. Te increase brings it up from 4400 to just shy of 5000.

BSA Fluor pH8 emissions0-900.PNG

The graph above is the emission intensity over time for our 0-900 second scans. As we have seen in previous trials, this is where largest change in emission intensity occurs around our wavelength of interest. The emission intensity jumps from approximately 10 to over 30 in the 900 seconds span. There also appears to be a minor shift to the right as the experiment went on.

BSA Fluor pH8 emissions1080-4500.PNG

The graph above is the emission intensity over time for our 1080-4500 second scans. Change in emission intensity around the peak has decreased, but the peak is not as refined as it is in the 4680-10800 graph.

BSA Fluor pH8 emissions4680-10800.PNG

The graph above is the emission intensity over time for our 4680-10800 second scans. This graph displays that there is minimal changes in the emission intensity in the area of our interest. The peak has become more refined through the course of our run, as well as through the extended inclusion of scans.