User:OdobaOkwuosa

Odoba Okwuosa

Contact Information

Email Address (ookwuosa at lion dot lmu dot edu)

Email OdobaOkwuosa through OpenWetware

Education

• Loyola Marymount University
  • Bachelor of Science Candidate, Biology major, Chinese Minor
  • Expected graduation: May 2024

Career Interests and Goals

• I am interested in genetics and epidemiology.

Work Experience

• Women’s Business Development Council （2020 - Present)
  • Special Projects Assistant
    • Coordinate and initiate fundraising to fund programs such as business literacy classes
    • Establish and maintain donor relations

Personal Interests/Hobbies

• My favorite things to do include cooking and traveling. Exposure to new things and places is very important in being a well-rounded person.

Favorite Aspect of Biology

• My favorite aspect of Biology is the variety it has, it is hard to get bored when there are so many fields to be studied under the large scope of it all.

Favorite Aspect of Computer Science

• My favorite part of Computer Science is the easy availability to information it provides. It improves the research process, and allows for efficiency to be increased

Dahlquist Lab Notebook

Fall 2021

Week 1

• Micropipetting Technique Practice

Week 2

• Micropipetting Technique Practice

Week 3

• Restriction Digest of Lambda DNA

Week 4

--

Week 5

• Lactase Persistence Protocol Run-through
  • Control PCR

Week 6

• Lactase Persistence Protocol Run-through
  • Gel Electrophoresis PCR Product Detection
  • Restriction Digest

Week 7

• Lactase Persistence Protocol Run-through
  • RFLP Analysis
  • Hair follicle DNA extraction

Week 8

• Hair follicle DNA extraction, PCR, and RFLP

Week 9

• PCR Re-run
• Hair follicle DNA extraction, PCR

Week 10

• PCR Product Purification, and Restriction Digest

Week 11

• PCR

Spring 2022

Week 1

• Protocol Optimization
  • 5% Chelex vs 10% Chelex (Set at 50 microliter)
  • 25 microliter PCR volume vs 50 microliter PCR volume (Set at 50 microliter)

Week 2

• Protocol Optimization (PCR)
  • PCR Amplification

Week 3

• --

Week 4

• --

Week 5

• DNA Hair Extraction

Week 6

• RFLP Analysis

Week 7

• --

Week 8

• RFLP Analysis

Week 9

• --

Week 10

• Mass PCR Purification
• RFLP Analysis

Week 11

• --

Week 12

• RFLP Analysis

Summer 2022

Week 1

• Hair DNA extraction, PCR (neg control contamination)
• Negative control PCR Re-run (2x)
• Reagent Replacement PCR Re-run

Week 2

• Reagent Replacement PCR Re-run
• Protocol optimization
  • PCR Gradient (best annealing temp - ~64-65 degrees)
• PCR Re-run

Week 3

• PCR Re-run
  • No plasmids - possible source of contamination
  • Negative control still contaminated
• PCR Purification

Week 4

• Restriction Digest
• DNA Extraction

Week 5

• DNA Extraction

Fall 2022

Week 1

• PCR

Week 2

• PCR

Week 3

• PCR

Week 4

• PCR

Week 5

• PCR

Week 6

• PCR

Week 7

• PCR

Week 8

• PCR

Week 9

• PCR

Week 10

• Summary & Organization of PCR
• PCR Purification & RFLP (Controls)

Week 11

• PCR Purification & RFLP

Week 12

• RFLP Analysis

Week 13

• PCR Purification

Week 14

• RFLP Analysis; PCR Purification

Week 15

• RFLP Analysis

Spring 2023

Week 1

• PCR Purification & RFLP Analysis

Week 2

• PCR Purification & RFLP Analysis

Acknowledgements

1. I used the user pages of Madeleine King and Annika Adinulos as samples of a user page
2. Instructions of Biol 368-01 Week 1 helped in formatting my headshot and user page.

References

OpenWetWare. (2020). BIOL368/S20:Week 1. Retrieved January 19, 2021, from https://openwetware.org/wiki/BIOL368/S20:Week_1