User:Noah Benjamin/Notebook/Experimental Biological Chem/2011/10/04

Biomaterials Design Lab

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Entry title Insert content here... Objective Description Repetition of "protein blank spectrum" from the Maltose Binding Protein lab. A UV-Vis Spectrum was taken of 'theoretical' MBP stock concentrations of 1000µg/mL, dilutions of 0.2, 2.0, 10, 100, and 1000 µL/mL. Using the equation solved in the full MBP/Bradford Reagent lab and the UV-Vis spectrum of the '2µg/mL' solution, the original stock concentration was solved to be 266.055µg/mL by back calculating from the true concentration of the '2µg/mL' solution. Data Equation used from previous experiment: At 500 nm, Absorbance= 0.231 x Concentration Resolved Concentrations: Dilutions: 0.53211, 0.053211, 2.66055, 26.6055, and 266.055 µg/mL Stock: 266.055 µg/mL -Peak is noticeable around 280 nm wavelength. Notes This area is for any observations or conclusions that you would like to note. Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.