User:Nancy T. Miles/Notebook/N.Miles Lab 2013-09-03/2013/09/03

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Objective

To determine the molar absorptivities of adenosine using UV-Vis spectra of varying known concentrations

Methods

The procedures to this experiment were taken from User:Matt_Hartings/Notebook/AU_Biomaterials_Design_Lab/2013/09/03.

From original stock solutions of 0.0083M adenosine and 0.0079M inosine, the following 1mL of dilutions were made:

Adenosine solution concentrations (M) Inosine solution concentrations (M)
3.00x10-5 4.80x10-5
2.50x10-5 4.00x10-5
2.00x10-5 3.20x10-5
1.50x10-5 2.40x10-5
1.00x10-5 1.60x10-5
0.50x10-5 0.80x10-5
0.25x10-5 (Random) 0.40x10-5 (Random)

Cuvettes were used to measure the UV-Vis Absorbance of the different concentrations of the diluted samples and a calibration curve was created.

Data

The following graph represents the absorbances of adenosine at the varying concentrations. 20130903 adenosineAbs 2.png

The following is the calibration curve of the corrected absorbance values of adenosine at the peak absorbance wavelength of 259nm. 20130903 adenosineCali.png