User:Nancy T. Miles/Notebook/N.Miles Lab 2013-09-03/2013/09/03

Biomaterials Design Lab

Objective

To determine the molar absorptivities of adenosine using UV-Vis spectra of varying known concentrations

From original stock solutions of 0.0083M adenosine and 0.0079M inosine, the following 1mL of dilutions were made:

Adenosine solution concentrations (M)	Inosine solution concentrations (M)
3.00x10^-5	4.80x10^-5
2.50x10^-5	4.00x10^-5
2.00x10^-5	3.20x10^-5
1.50x10^-5	2.40x10^-5
1.00x10^-5	1.60x10^-5
0.50x10^-5	0.80x10^-5
0.25x10^-5 (Random)	0.40x10^-5 (Random)

Cuvettes were used to measure the UV-Vis Absorbance of the different concentrations of the diluted samples and a calibration curve was created.

The following graph represents the absorbances of adenosine at the varying concentrations.

The following is the calibration curve of the corrected absorbance values of adenosine at the peak absorbance wavelength of 259nm.