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Function an importance of the chemicals used in the different suspensions and systems
This entry contains the information on the roles played by each single chemical in the suspensions and systems we use for our experiments. IT IS NOT COMPLETE YET.
The Glucose Oxidase oxidizes the D-Glucose, which serves as fuel in the system. In this process it uses all the O2 present, which is the causing agent for photobleaching. As this process takes place, H202 is formed.
The Catylase decomposes the produced H202.
The BME prevents blinking of the fluorophore.
PIPES is the buffering agent.
EGTA takes care of the Ca+ excess
MgCl2 contains Mg, which presences is vital for the motility.
NaOH is used as the pH agent and to grant solubility