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α – PEM
The concentration of α-Casein in PEM has to be 1 mg/ml. In this particular case, we prepared a solution with a total volume of 26 ml and since we have the 10xPEM buffer stored, the amounts of compounds needed are:
Place all three compounds in an autoclaved beaker. Place a stirring magnet in the beaker and stir the solution for 45 minutes to an hour.
Once ready, prepare 1 ml aliquots and store them in the 4C fridge. Label the vials in the following manner: α-PEM (1 mg/ml α-Casein in PEM)
α-PEM (1 mg/ml α-Casein in PEM) is different from PEM-α (0.5 mg/ml α-Casein in PEM)