User:Mischa L Spiegel/Notebook/The effects of DMSP on angiogenesis/2015/07/02

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Day 3

  • 48 wells coated with 50µl gelatin (1%)and incubated for 1hour at 37oc on a 96 well plate
  • Trypsinised and resuspended cells
  • Counted 5µl cells + 5µl trypan blue in haemocytometer (average=17cells) x2 =34 x10,000 = 3.4x10^4 cells per ml
  • Set up serial dilutions of 16,000; 8,000; 4,000; 2,000; 1,000 cells in 96 well plate with 4 replicates of each