User:Mischa L Spiegel/Notebook/The effects of DMSP on angiogenesis/2015/07/03

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Day 4

  • Stained cells from serial dilutions in 96 well plate with methylene blue and read at A610
  • Found that cells of 16,000 and 8,000 did not grow well or adhere
  • Not much difference between 4,000; 2,000; 1,000
  • Chose to begin with 2,000 for room to grow- for proliferation studies next week

Thawed WT(U1)8 cells P13 in 10% DMSO, 90% serum and put into cell culture for proliferation assays next week