User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/12/07

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Objective To test the procedures from 12/06/11 with different ratios of Au/BSA. Test ratios of 100 and 35. Description Create solutions for ratio of 100 Au:BSA Using 17.7 μM BSA stock solution and 2.5 mM HAuCl4 stock solution, combine 0.5 mL gold solution and 0.0706 mL BSA solution. Dilute with 5 mL of water. Repeat to make a second solution. Cover with tin foil. Create solutions for ratio of 35 Au: BSA Using the same stock solutions, combine 0.5 mL gold solution and 2.02 mL BSA solution. Dilute with 5 mL of water. Repeat to make a second solution. Cover with tin foil. Put all solutions into the oven at about 75°C. Leave one of each ratio concentration in for the duration of lab (about 3 hours). Take one of each ratio concentration out for 10 minutes every half hour for 3 hours. Data Solution 1: Au/BSA = 35, left in the oven Solution 2: Au/BSA = 35, taken out of the oven every 30 minutes Solution 3: Au/BSA = 100, left in the oven Solution 4: Au/BSA = 100, taken out of the oven every 30 minutes Notes