User:Michaela Harper/Notebook/Biological Chemistry Lab/2011/08/31
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To use BSA (bovine serum albumin) to synthesize gold nanoparticles. BSA reduces Au3+ to Au 0 for the synthesis to occur.
This graph shows the absorbance for each spectrum taken every 30 minutes. First, a blank spectrum of the buffer solution was taken and subracted from each of the following spectra to create a corrected absorbance. Series 1 represents the spectra taken at t=0, series 2 represents the spectra taken at t=30 minutes, etc up to series 6 representing t=150 minutes.
I would also like to see this graph zoomed in to the features at 550. The thing I would like to know is: Are the changes at 550nm due to a new feature in the spectrum or just an increase in the baseline<b> Matt Hartings 08:53, 6 September 2011 (EDT)
This graph shows the corrected absorbance at 550nm over time. This represents the concentration of gold nanoparticles that were formed. However, from the zoomed in graph, it is apparent that the changes are due to an increase in the baseline.
This graph shows the zoomed in features at 550nm. The changes at 550nm are due to an increase in the baseline, not a new feature.
The color of the mixture turned a very slight yellow over time, then returned to a clear color. The UV-Vis spectra were taken from 200-800nm using a 1mL quartz cuvette.